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Chapter 4

🧬 Principles of Inheritance and Variation Study Notes

Mendel · crosses · linkage · sex determination · genetic disorders

Chapter Content: Study Notes MCQ Practice Flashcards

4.1 1 · Genetics, Heredity & Variation

Genetics is the branch of biology that studies the principles of inheritance and its practices.

  • Heredity — transmission of characters (resemblances) from parents to offspring.
  • Variation — the degree by which offspring differ from their parents and among themselves.
Big idea: Every sexually reproducing organism shows both heredity (why children resemble parents) and variation (why they are never identical).

Humans knew inheritance from ~8000–1000 BC (domestication, breeding) but the scientific basis was given by Gregor Johann Mendel (1856–1863) through pea-plant experiments.

4.2 2 · Mendel & His Pea-Plant Experiments

Mendel worked on the garden pea (Pisum sativum) and analysed results mathematically/statistically — a first in biology. He studied 7 pairs of contrasting traits.

Why the pea plant was ideal

  • Many distinct, contrasting characters with clear-cut forms.
  • Bisexual flowers that are naturally self-pollinating (true-breeding lines).
  • Could be easily artificially cross-pollinated (emasculation + dusting pollen).
  • Short life cycle, easy to grow, produces large numbers of offspring.

The 7 contrasting traits Mendel selected

CharacterDominantRecessive
Stem heightTallDwarf
Flower colourVioletWhite
Flower positionAxialTerminal
Pod shapeInflated (full)Constricted
Pod colourGreenYellow
Seed shapeRoundWrinkled
Seed colourYellowGreen
True-breeding: a line that, on self-pollination, shows the stable trait over many generations.

4.3 3 · Key Vocabulary

TermMeaning
Factor / GeneUnit of inheritance controlling a character (Mendel's "factor").
AlleleSlightly different forms of the same gene (e.g. T and t).
GenotypeGenetic constitution (e.g. Tt).
PhenotypeObservable trait (e.g. Tall).
HomozygousIdentical alleles (TT or tt).
HeterozygousDifferent alleles (Tt).
DominantAllele expressed in heterozygote (capital, T).
RecessiveAllele masked in heterozygote (small, t).

Convention: capital letter = dominant allele, same small letter = recessive allele.

4.4 4 · Monohybrid Cross & Laws of Dominance/Segregation

A cross tracking one pair of contrasting traits.

Tall TT × Dwarf tt (P) F₁ : all Tall (Tt)→ self F₂ : 3 Tall : 1 Dwarf
  • F₂ phenotypic ratio = 3 : 1 (Tall : Dwarf).
  • F₂ genotypic ratio = 1 : 2 : 1 (TT : Tt : tt).
  • The Punnett square (by R. C. Punnett) predicts these combinations.

Mendel's Laws (from monohybrid)

Law of Dominance: Characters are controlled by discrete factors occurring in pairs; in a dissimilar pair one factor (dominant) expresses and masks the other (recessive).
Law of Segregation (Purity of Gametes): The two alleles of a pair separate during gamete formation so that each gamete carries only one allele; they do not blend and are passed on pure.

Test cross: cross the unknown dominant phenotype with a homozygous recessive to reveal its genotype (offspring ratio 1:1 → heterozygous; all dominant → homozygous). Back cross: cross F₁ with either parent.

4.5 5 · Incomplete Dominance & Co-dominance

FeatureIncomplete DominanceCo-dominance
F₁ phenotypeIntermediate (blend)Both alleles expressed together
ExampleSnapdragon / Mirabilis jalapa: Red × White → PinkHuman ABO blood group (Iᴬ & Iᴮ)
F₂ ratio1 : 2 : 1 (Red:Pink:White) — phenotype = genotype ratioDepends on cross; both antigens appear (AB)

ABO blood groups — multiple alleles + co-dominance

Gene I has three alleles: Iᴬ, Iᴮ, i (a case of multiple allelism — more than two alleles for one gene). Iᴬ and Iᴮ each make a surface sugar (antigen A / B); i makes none. Iᴬ and Iᴮ are dominant over i but co-dominant to each other.

Blood groupGenotype(s)
AIᴬIᴬ or Iᴬi
BIᴮIᴮ or Iᴮi
ABIᴬIᴮ (co-dominance)
Oii

4.6 6 · Dihybrid Cross & Independent Assortment

A cross tracking two pairs of traits together (seed shape + seed colour).

RRYY × rryy F₁ : RrYy (Round Yellow)→ self F₂ : 9 : 3 : 3 : 1
  • F₂ phenotypic ratio 9:3:3:1 = 9 Round Yellow : 3 Round Green : 3 Wrinkled Yellow : 1 Wrinkled Green.
  • New (recombinant) combinations appear (Round-Green, Wrinkled-Yellow).
Law of Independent Assortment: When two pairs of traits are combined in a hybrid, the segregation of one pair is independent of the other pair.

4.7 7 · Chromosomal Theory of Inheritance

Mendel's work was rediscovered in 1900 (de Vries, Correns, von Tschermak).

Walter Sutton & Theodore Boveri (1902) noticed that chromosome behaviour during meiosis parallels the behaviour of Mendel's factors, and united the two ideas as the Chromosomal Theory of Inheritance.

Chromosome behaviourGene / factor behaviour
Occur in pairs (homologues)Alleles occur in pairs
Segregate at gamete formationAlleles segregate (Law of Segregation)
Independent pairs assort independentlyIndependent Assortment

4.8 8 · Morgan, Linkage & Recombination

Thomas Hunt Morgan worked on the fruit fly Drosophila melanogaster and gave experimental proof of the chromosomal theory.

Why Drosophila is a good model

  • Grows on simple synthetic medium; life cycle ~2 weeks.
  • A single mating gives many progeny; clear sexual dimorphism.
  • Many hereditary variations visible under a low-power microscope.
LinkageRecombination
Physical association of genes on the same chromosome; tend to be inherited together.Generation of non-parental gene combinations (crossing over).
Genes close together → tightly linked, low recombination.Genes far apart → loosely linked, high recombination.

Morgan's Drosophila dihybrid data: genes for white eye & yellow body were tightly linked (~1.3% recombination); white eye & miniature wing showed ~37.2% recombination.

Alfred Sturtevant used recombination frequency between gene pairs to map their positions — the basis of genetic maps.

4.9 9 · Polygenic Inheritance & Pleiotropy

Polygenic inheritancePleiotropy
Many genes → one traitOne gene → many traits
Phenotype = additive effect of alleles + environment; shows a range/gradient.A single gene affects several, seemingly unrelated, characters.
Examples: human skin colour, height.Examples: phenylketonuria, sickle-cell anaemia, starch synthesis in pea (round/wrinkled).

In skin colour, the more dominant alleles present (e.g. of genes A,B,C), the darker the skin — a quantitative, continuous trait.

4.10 10 · Sex Determination

SystemFemaleMaleExample
XX–XOXXXO (one X, no Y)Grasshopper / many insects
XX–XYXX (homogametic)XY (heterogametic)Humans, Drosophila
ZW–ZZZW (heterogametic)ZZ (homogametic)Birds

Humans: 22 pairs of autosomes + 1 pair of sex chromosomes. Female = XX, Male = XY. Because the father is heterogametic (X- or Y-bearing sperm), the sex of the child is decided by the father.

Sex determination in honeybees — haplodiploidy

Fertilised egg (2n=32)Female (queen/worker)
Unfertilised egg (n=16)→ parthenogenesisMale (drone)
Consequence: males (drones) are haploid — they have no father and cannot produce sons (but have a grandfather and can have grandsons).

4.11 11 · Mendelian (Single-gene) Disorders

DisorderTypeKey facts
HaemophiliaX-linked recessiveA clotting-factor protein not made → non-stop bleeding; mostly affects males; classic royal pedigree (Queen Victoria).
Sickle-cell anaemiaAutosomal recessiveGlutamic acid → Valine at 6th position of β-globin (GAG→GUG); HbˢHbˢ affected, HbᴬHbˢ carrier; RBCs sickle at low O₂.
PhenylketonuriaAutosomal recessiveEnzyme converting phenylalanine → tyrosine is absent; phenylalanine + derivatives accumulate → mental retardation; excreted in urine.
ThalassemiaAutosomal recessiveReduced synthesis of globin chains (α on chr 16 / β on chr 11); a quantitative problem (too little globin). Contrast: sickle-cell is qualitative.
Colour blindnessX-linked recessiveRed-green defect; ~8% of males, ~0.4% of females.
Thalassemia vs Sickle-cell: Thalassemia = quantity defect (fewer globin chains); Sickle-cell = quality defect (abnormal globin).

4.12 12 · Chromosomal Disorders

Aneuploidy = gain/loss of a chromosome due to non-disjunction (failure of segregation). Polyploidy = gain of a whole chromosome set (common in plants).

DisorderKaryotypeFeatures
Down's syndromeTrisomy 21 (extra 21)Short stature, small round head, furrowed protruding tongue, partly open mouth, broad palm with a characteristic crease; retarded mental & physical development. (Described by Langdon Down.)
Klinefelter's syndrome47, XXY (extra X)Overall masculine but with some feminine development (gynaecomastia = breast development); sterile.
Turner's syndrome45, X0 (missing X)Sterile female; rudimentary ovaries; lack of other secondary sexual characters.

Pedigree analysis studies a trait across generations using standard symbols (□ male, ○ female, filled = affected), used for genetic counselling.

⚡ Mini-Review: Interactive Flashcards

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Question Who is the 'Father of Genetics'?
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Answer Gregor Johann Mendel, from his pea-plant work (1856–1863).
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